描述:Cygnus technologies, Inc.是美國一家專門從事生物工程和制藥過程中殘留物檢測的一系列產(chǎn)品。生物制品生產(chǎn)過程中存在許多影響產(chǎn)品質(zhì)量的殘留物,諸如特殊重組表達系統(tǒng)中宿主細胞蛋白( Host Cell protein, HCP )的殘留以及生物制品加工過程中培養(yǎng)物殘留的污染物。公司提供了廣泛而靈敏度*的一系列用于檢測生物工程殘留物的產(chǎn)品,
1.CHO HCP ELISA kit(F015)
zui常用的用于檢測中國倉鼠卵巢細胞宿主蛋白試劑盒。
1)CHO HCP ELISA kit(F015)盒子組成
2)操作流程(中文操作流程僅供參考,實驗請參照原裝英文說明書)
客戶可以根據(jù)檢測靈敏度的需要,選擇其中一種檢測方法。
兩種操作方法的比較:
Rapid protocol | High sensitivity protocol | |
標準品 | 0,4,20,75,250ng/ml | 0,1,4,20,75ng/ml |
檢測耗時 | 2+1.5=3.5h | 2+2+1.5=5.5h |
靈敏度 | 2 ng/ml | <500pg/ml |
The antibodies used in the kit were generated against and affinity purified from a cell lysate of CHO cells grown in serum supplemented media. All three of the Cygnus CHO HCP kits recognize a qualitatively vast majority of the same HCPs. The potential exists that the quantitative value reported for a given sample type may differ between the kits, therefore we cannot predict which kit will be most suitable for your applications. Formal qualification must be performed on each different product, to determine which of the 3 kits are superior. For labs looking at CHO HCP contamination for the first time, we suggest evaluating our CHO HCP ELISA, 3G, Catalog # F550. If this method proves inadequate then we suggest evaluating CHO HCP kits, Catalog #CM015 and F015.
Catalog # | Size | Price | Qty. |
---|---|---|---|
F015 | 1 kit | 9375 |