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彈性蛋白酶 Elastase

描述:彈性蛋白酶 Elastase 來源于豬胰臟

更新時間:2017-12-19
訪問次數(shù):2522
廠商性質(zhì):代理商
詳情介紹

彈性蛋白酶 Elastase

彈性蛋白酶 Elastase 來源于豬胰臟,分子質(zhì)量25.9KD,PH8.5.彈性蛋白酶水解蛋白底物種類繁多,但也有其*它能夠水解彈性蛋白,胰蛋白酶,糜蛋白酶胃蛋白酶不能作用的底物。大豆胰蛋白酶抑制劑激肽釋放酶抑制劑抑制蛋白水解,但無彈性蛋白離解活性。

穩(wěn)定性/儲存彈性蛋白酶pH值3.5不穩(wěn)定。
2-8° C干粉穩(wěn)定貯存6-12個月

彈性蛋白酶產(chǎn)品代碼ES和ESL中性pH值濃度大于0.25%時溶解性差。當(dāng)用反應(yīng)混合物或培養(yǎng)基稀釋彈性蛋白酶之前,調(diào)整kcl或堿性緩沖液的離子強(qiáng)度或PH值有助于彈性蛋白酶的溶解,當(dāng)pH4.0-10.4穩(wěn)定。

技術(shù)說明:1 SucAla3NA單位大約相當(dāng)于6倍的彈力蛋白酶的消化單位應(yīng)用無菌水進(jìn)行溶解,避免細(xì)菌污染。由于懸浮液粘稠性代號:ES的小瓶包裝)應(yīng)用水沖洗以保持濃度。

單位定義
25 ° C,pH值8.0,1單位的酶每分鐘切開1微摩爾N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide的量。

Description Activity Code Size Cat. No. Price  
Elastase, Purified
Chromatographically purified. A lyophilized powder.
Store at 2-8° C.
REQUIRES SPECIAL SHIPPING: ICE PACK
≥8 units per mg protein ESFF 5 mg LS006363    
20 mg LS006365  
Bulk LS006367 Inquire
Elastase, Lyophilized
2X Crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells.
Store at 2-8° C.
≥3 units per mg protein ESL 25 mg LS002290      
100 mg LS002292    
1 gm LS002294    
Bulk LS002298 Inquire
Elastase, Suspension
2X crystallized. Supplied as an aqueous suspension. This preparation must be diluted to dissolve the enzyme. The diluted enzyme should be 0.22 micron filtered before use. Suitable for the isolation of Type II lung cells.
Store at 2-8° C. DO NOT FREEZE
REQUIRES SPECIAL SHIPPING: ICE PACK.
≥3 units per mg protein ES 25mg LS002274    
100mg LS002279    
1 gm LS002280    
Bulk LS002276 Inquire

 

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