彈性蛋白酶 Elastase
彈性蛋白酶 Elastase 來源于豬胰臟,分子質(zhì)量25.9KD,PH8.5.彈性蛋白酶水解的蛋白底物的種類繁多,但也有其*性,它能夠水解彈性蛋白,胰蛋白酶,糜蛋白酶或胃蛋白酶不能作用的底物。大豆胰蛋白酶抑制劑和激肽釋放酶抑制劑抑制蛋白水解,但無彈性蛋白離解活性。
穩(wěn)定性/儲存:彈性蛋白酶在pH值≤3.5時不穩(wěn)定。2-8° C干粉穩(wěn)定貯存6-12個月。
彈性蛋白酶產(chǎn)品代碼:ES和ESL在中性pH值和在濃度大于0.25%時溶解性差。當(dāng)用反應(yīng)混合物或培養(yǎng)基稀釋彈性蛋白酶之前,調(diào)整kcl或堿性緩沖液的離子強(qiáng)度或PH值有助于彈性蛋白酶的溶解,當(dāng)pH4.0-10.4時穩(wěn)定。
技術(shù)說明:1 SucAla3NA單位大約相當(dāng)于6倍的彈力蛋白酶的消化單位。應(yīng)用無菌水進(jìn)行溶解,以避免細(xì)菌污染。由于懸浮液的粘稠性(代號:ES的小瓶包裝)應(yīng)用水沖洗以保持濃度。
單位定義:25 ° C,pH值8.0,1單位的酶每分鐘切開1微摩爾的N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide的量。
Description | Activity | Code | Size | Cat. No. | Price | ||
---|---|---|---|---|---|---|---|
Elastase, Purified Chromatographically purified. A lyophilized powder. Store at 2-8° C. REQUIRES SPECIAL SHIPPING: ICE PACK | ≥8 units per mg protein | ESFF | 5 mg | LS006363 | |||
20 mg | LS006365 | ||||||
Bulk | LS006367 | Inquire | |||||
Elastase, Lyophilized 2X Crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. Store at 2-8° C. | ≥3 units per mg protein | ESL | 25 mg | LS002290 | |||
100 mg | LS002292 | ||||||
1 gm | LS002294 | ||||||
Bulk | LS002298 | Inquire | |||||
Elastase, Suspension 2X crystallized. Supplied as an aqueous suspension. This preparation must be diluted to dissolve the enzyme. The diluted enzyme should be 0.22 micron filtered before use. Suitable for the isolation of Type II lung cells. Store at 2-8° C. DO NOT FREEZE REQUIRES SPECIAL SHIPPING: ICE PACK. | ≥3 units per mg protein | ES | 25mg | LS002274 | |||
100mg | LS002279 | ||||||
1 gm | LS002280 | ||||||
Bulk | LS002276 | Inquire |