Gene Bridges GmbH,成立于2000年,是一家從歐洲分子生物學(xué)實(shí)驗(yàn)室(EMBL)分離出來的獨(dú)立經(jīng)營的公司,主要經(jīng)營其產(chǎn)品Red/ET重組技術(shù)的試劑盒。 Red/ET重組是新近出現(xiàn)的一種基于λ噬菌體Red操縱子(Redα/Redβ/Redγ)和Rac噬菌體RecE/RecT重組系統(tǒng)的DNA工程技術(shù)。 通過該技術(shù)可以簡單、快速地對(duì)任意大的DNA分子進(jìn)行插入、敲除、突變等多種修飾,而且還可對(duì) 長達(dá)80kb的DNa片段進(jìn)行亞克隆。由于重組反應(yīng)的整個(gè)過程都是在大腸桿菌細(xì)胞內(nèi)部完成,因此不存在堿基突變危險(xiǎn)。該技術(shù)已被廣泛地用于基因組DNA, 如細(xì)菌人工染色體、大腸桿菌染色體等的遺傳修飾研究以及基因工程藥物的研究和開發(fā)中。 Cat# | Name | Size | Price | K004 | Quick & Easy Red/ET Conditional Knock Out Kit - FRT (need license to buy) | Kit | 15249 | K005 | Quick & Easy Red/ET Conditional Knock Out Kit - loxP (need license to buy) | Kit | 15249 | K006 | Quick & Easy Red/ET E. coli Gene Deletion Kit (need license to buy) | Kit | 13039 | A008 | Red/ET Ampicillin Selection Cassette, FRT-flanked (FRT-amp-FRT) | 50ng/ul,20ul | 6077.5 | A009 | Red/ET Ampicillin Selection Cassette, loxP flanked (loxP-amp-loxP) | 50ng/ul,20ul | 6077.5 | K003 | Red/ET BAC Subcloning Kit (need license to buy) | Kit | 13260 | A006 | Red/ET Chloramphenicol Selection Cassette, FRT-flanked (FRT-cm-FRT) | 50ng/ul,20ul | 6077.5 | A007 | Red/ET Chloramphenicol Selection Cassette, loxP-flanked (loxP-cm-loxP) | 50ng/ul,20ul | 6077.5 | K002 | Red/ET Counter Selection BAC Modification Kit (need license to buy) | Kit | 17459 | A112 | Red/ET Cre Expression Plasmid:705-Cre, cm-selection | 0.2ug/ul,20ul | 6077.5 | A113 | Red/ET Cre Expression Plasmid:706-Cre, tet-selection | 0.2ug/ul,20ul | 6077.5 | A201 | Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Academic price, requires MTA) | 0.2ug/ul,20ul | 8287.5 | A202 | Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Industry price, requires MTA) | 0.2ug/ul,20ul | 71825 | A104 | Red/ET Enhanced Flp Expression Plasmid:707-FLPe, tet-selection | 0.2ug/ul,20ul | 6077.5 | A105 | Red/ET Enhanced Flp Expression Plasmid:708-FLPe, cm-selection | 0.2ug/ul,20ul | 6077.5 | A010 | Red/ET Hygromycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-hygro-FRT) | 50ng/ul,20ul | 6077.5 | A011 | Red/ET Hygromycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-hygro-loxP) | 50ng/ul,20ul | 6077.5 | A005 | Red/ET Neomycin Selection Cassette plus loxP site 2nd version, FRT flanked, Prokaryotic and Eukaryotic (loxP-FRT-PGK-gb2-neo-FRT) | 50ng/ul,20ul | 6077.5 | A004 | Red/ET Neomycin Selection Cassette plus loxP site, FRT flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-neo-FRT-loxP) | 50ng/ul,20ul | 6077.5 | A002 | Red/ET Neomycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-neo-FRT) | 50ng/ul,20ul | 6077.5 | A003 | Red/ET Neomycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-neo-loxP) | 50ng/ul,20ul | 6077.5 | A001 | Red/ET Neomycin Selection Cassette, Prokaryotic and Eukaryotic (PGK-gb2-neo) | 50ng/ul,20ul | 6077.5 | K001 | Red/ET Quick & Easy BAC Modification Kit (need license to buy) | Kit | 14807 |
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