描述:BugBuster蛋白抽提試劑用于溫和破壞大腸桿菌細胞壁以釋放活性蛋白。作為激烈的機械法(如弗式壓榨法和超聲波法)的替代方法,這種試劑處理起來非常簡便、快速,且成本低廉,適合制備表達蛋白用于下游的純化等應用。
Product | Size | Cat.No. | Price(¥) |
BugBuster® Protein Extraction Reagent | 100 ml | 70584-3 | 750.00 |
500 ml | 70584-4 | 3,789.00 | |
BugBuster® Master Mix | 100 ml | 71456-3 | 1,700.00 |
500 ml | 71456-4 | 5,200.00 | |
CytoBuster™ Protein Extraction Reagent | 50 ml | 71009-3 | 824.00 |
250 ml | 71009-4 | 3,122.00 |
Comparison of E. coli lysis methods 50 ml samples of an induced 500 ml culture of BL21(DE3) containing pET-41a(+) encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity. |
Product | Size | Cat.No. | Price(¥) |
Benzonase® Nuclease, Purity > 90% | 10 ku | 70746-3 | 1,200.00 |
2.5 ku | 70746-4 | 400.00 |
Viscosity reduction by Benzonase Nuclease E. coli BL21(DE3) cells containing a pET construct were suspended in BugBuster Reagent (5 ml/g wet weight). Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at |